Affiliation:
1. Animal Pathology Division, Canada Department of Agriculture, Hull, Quebec, Canada
Abstract
Sphaerophorus necrophorus
(bovine liver abscess isolates) antiserum was fractionated and labeled with fluorescein isothiocyanate. The fluorescent-antibody (FA) conjugate proved to be species-specific and facilitated the detection of
S. necrophorus
cells in liver abscesses, viscera, and ruminal contents of cattle. Brightly fluorescing
S. necrophorus
cells were observed in experimentally inoculated soil incubated anaerobically at 37 and 4 C for 8 and 10 months, respectively. When incubated under moist conditions (80% water holding capacity) at 37 C, the test organism survived in both sterile and unsterile soil for as long as 8 weeks. Results strongly support the feasibility of using FA techniques concurrent with cultural methods for rapid detection of
S. necrophorus
infections.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference16 articles.
1. Batty I. 1967. Fluorescenit-labelled antibodies as tools in the detection identification and differentiation of clostridial species p. 85-99. In V. Fredette (ed.) The anaerobic bacteria. The Institute of Microbiology and Hygiene Univ. of Montreal.
2. A study of twelve strains of Bacillus ntecraphorus with observations of the oxygen intolerance of the organism;Beveridge W. I. B.;J. Pathol. Bacteriol.,1934
3. Nonispecific staining: its control in immunofluorescence examination of soil;Bohlool B. B.;Science,1968
4. Breed R. S. E. G. D. Murray and N. R. Smith. 1957. Bergey's manual of determinative bacteriology 7th ed. The Williams and Wilkins Co. Baltimore.
5. Isolation of Sphaerophorus necrophorus from bovine liver abscesses;Calkins H. E.;Appl. Microbiol.,1967
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献