Affiliation:
1. The International Rice Research Institute, Los Baños, Laguna, Philippines
Abstract
Acetylene reduction activity of intact rice plants was measured in closed assay chambers with plants grown in water culture. Acetylene was added to the liquid medium, and the ethylene formed was measured from both gas and liquid phases. After cutoff of mineral nitrogen supply and inoculation of fresh soil, rice plants grown from the seedling stage in water culture exhibited acetylene reduction activity after a lag period. However, rice plants grown in a paddy field and transferred to water culture were more suitable for N
2
fixation studies because of their higher, less variable acetylene reduction activity. The time course of acetylene reduction was monitored by continuous circulation of gas between the gas phase and the liquid phase, and the result showed an initial 2- or 3-h period of lower activity, followed by increased and almost constant activity up to 24 h. The effects on acetylene reduction activity of aeration, ammonium, chloramphenicol, and 3-(3,4-dichlorophenyl)-1,1-dimethylurea addition are reported. Ammonium was inhibitive at 0.33 mM, and its depressive effect was alleviated by ammonium uptake by the plants.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference32 articles.
1. Assaying nitrogenase (C2H2) activity in the field;Balandreau J.;Bull. Ecol. Res. Commun. Stockholm,1973
2. Balandreau J. P. G. Rinaudo M. M. Oumarov and Y. R. Dommergues. 1975. Asymbiotic N2 fixation in paddy soils p. 611-628. In W. E. Newton and C. J. Nyman (ed.) 1st International Symposium on Nitrogen Fixation. Washington State University Press Pullman Washington.
3. Acetylene reduction (nitrogen fixation) associated with corn inoculated with;Barber L E;Spirillum. Appl. Environ. Microbiol.,1976
4. Nitrogenase activity of the Paspalum notatum-Azotobacter paspali association and oxygen sensitivity;Dobereiner J.;J. Gen. Microbiol.,1971
5. Non-symbiotic nitrogen fixation in the rhizosphere of rice, maize, and different tropical grasses;Dommergues Y.;Soil Biol. Biochem.,1973
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