Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay-Reference-Cited by-同舟云学术

Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay

Published:1991-09 Issue:9 Volume:29 Page:2056-2058
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Knowles D P¹,Perryman L E¹,Kappmeyer L S¹,Hennager S G¹

Affiliation:

1. Animal Disease Research Unit, USDA-ARS, Pullman, Washington 99164-7030.

Abstract

A competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) was developed to detect antibody to Babesia equi. One hundred fifty-four equine serum samples from 19 countries were tested for antibody to B. equi by the complement fixation test and by CI ELISA. The CI ELISA and complement fixation test results agreed in 94% (144) of the serum samples tested. The 10 discrepant serum samples were retested and analyzed for ability to immunoprecipitate in vitro translation products from B. equi merozoite mRNA. Five discrepant results were clearly resolved in favor of the CI ELISA, and the remaining five discrepancies were not definitively resolved.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.29.9.2056-2058.1991

Reference11 articles.

1. Use of monoclonal antibody in a blocking ELISA to detect group specific antibodies to bluetongue virus;Anderson J.;J. Immunol. Methods,1984

2. Equine piroplasmosis: production of antigens for the complement fixation test;Frerichs W. M.;Am. J. Vet. Res.,1969

3. Equine piroplasmosis: complement fixation titers of horses infected with Babesia caballi;Frerichs W. M.;Am. J. Vet. Res.,1969

4. The piroplasms of Equidae-significance for international commerce;Friedhoff K. T.;Berl. Muench. Tieraerztl. Wochenschr.,1982

5. Studies on the complement fixation reaction for equine piroplasmosis;Hirato K.;Jpn. J. Vet. Sci.,1945

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