Reproducibility of tolerance tests that are useful in the identification of campylobacteria

Abstract

Twenty type or other reference strains, each representing a different Campylobacter, Helicobacter, or Arcobacter taxon, were used to assess the reproducibility of 25 phenotypic tests that are used in the identification of such organisms. Twenty-two of the tests depended on growth inhibition, and each of these tolerance tests was performed by using three different basal media. Although the overall reproducibility of the tests with each basal medium exceeded 89%, the proportion of strains that were able to grow in a reproducible manner on the basal media varied from 100% for blood agar and 50% for nutrient agar to 5% for brucella agar. In general, test reproducibility was highest with the basal medium that supported the most luxuriant growth. For the majority of tests, the basal medium which gave the optimum reproducibility could be determined.