Assay for detection of Trypanosoma cruzi antibodies in human sera based on reaction with synthetic peptides-Reference-Cited by-同舟云学术

Assay for detection of Trypanosoma cruzi antibodies in human sera based on reaction with synthetic peptides

Published:1991-09 Issue:9 Volume:29 Page:2034-2037
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Vergara U¹,Lorca M¹,Veloso C¹,Gonzalez A¹,Engstrom A¹,Aslund L¹,Pettersson U¹,Frasch A C¹

Affiliation:

1. Departamento de Ciencias Biologicas y Preclincos, Facultad de Medicina, Universidad de Chile, Santiago.

Abstract

Synthetic peptides modelled according to the amino acid sequences derived from the repeated domains of five Trypanosoma cruzi antigens were used in an immunoradiometric assay to detect antibodies appearing after natural human infections. An enzyme-linked immunosorbent assay and an indirect immunofluorescence assay performed with a complex antigenic mixture from parasites were used as controls. The results indicate that the synthetic peptides were recognized by a large proportion of serum samples collected from 34 patients with Chagas' disease in Chile and point to their possible use in diagnosis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.29.9.2034-2037.1991

Reference19 articles.

1. Identification of a Trypanosoma cruzi antigen that is shed during the acute phase of Chagas' disease;Aifranchino J. L.;Mol. Biochem. Parasitol.,1989

2. Fluorescent antibody test for the serodiagnosis of American trypanosomiasis. Technical modification employing preserved culture forms of Tr-vpanosorna cruzi in slide test;Camargo M. E.;Rev. Inst. Med. Trop. Sao Paulo,1966

3. Camargo M. E. and G. F. K. Takeda. 1979. Diagnostico de laboratorio p. 175-198. In Z. Brener and Z. Andrade (ed.) Trypanosoina cruzi e Doenca de Chagas. Guanabara Koogan Brazil.

4. Antibodies to the repetitive epitope of Plasmodium falciparum circumsporozoite protein in a rural Tanzanian community: a longitudinal study of 132 children;Del Giudice G.;Am. J. Trop. Med. Hyg.,1987

5. Diagnosis of Chagas disease using recombinant DNA technology;Frasch A. C. C.;Parasitol. Today,1990

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