Affiliation:
1. Department of Virology, Agricultural University Wageningen, P.O. Box 8045, 6700 EM Wageningen, and Center for Plant Breeding Research, P.O. Box 16, 6700 AA Wageningen, 2 The Netherlands
Abstract
Baculoviruses are insect pathogens with a relatively slow speed of action, and this has limited their use as control agents of insect pests. Introduction into baculoviruses of genes which code for proteins interfering specifically with insect metabolism or metamorphosis, such as toxins, hormones, and enzymes, may enhance the pathogenicity of these viruses. The complete insecticidal crystal protein gene
cryIA(b)
of
Bacillus thuringiensis
subsp.
aizawai
7.21 was engineered into the nuclear polyhedrosis virus of
Autographa californica
(AcNPV) in place of the polyhedrin gene. In infected
Spodoptera frugiperda
cells, the
cryIA(b)
gene was expressed at a high level without interference with AcNPV production. The crystal protein was found in the cytoplasm of
S. frugiperda
cells, mainly as large crystals with an ultrastructure similar to that of
B. thuringiensis
crystals. Infected-cell extracts inhibited feeding of the large cabbage white
Pieris brassicae.
The toxicity of the crystal protein expressed by AcNPV recombinants was comparable with that of the crystal protein expressed by a corresponding
Escherichia coli
recombinant.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference36 articles.
1. A rapid alkaline extraction procedure for screening recombinant plasmid DNA;Birnboim H. C.;Nucleic Acids Res.,1979
2. Genetic engineered viral insecticides. A progress report. 1986-1989;Bishop D. H. L.;Pestic. Sci.,1989
3. Characterization of the entomocidal parasporal crystal of Bacillus thuringiensis;Bulla L. A.;J. Bacteriol.,1977
4. Entwistle P. F. and H. F. Evans. 1986. Viral control p. 347-412. In G. A. Kerkut and L. I. Gilbert (ed.) Comprehensive insect physiology biochemistry and pharmacology vol. 13. Pergamon Press Inc. Elmsford N.Y.
5. Finney D. J. 1962. Probit analysis a statistical treatment of the sigmoid response curve. Cambridge University Press Cambridge.
Cited by
62 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献