Comparison of Murine Sarcoma Viruses in Nonproducer and S + L − -Transformed Cells

Abstract

The biologic properties of murine sarcoma viruses in nonproducer and S + L − cells were compared. Each virus was found to be genetically stable through at least two cycles of rescue and transmission to new cells. There was no evidence for production of a defective murine leukemia virus by S + L − cells. The results are most consistent with the hypothesis that the sarcoma genome in S + L − cells contains information for replication functions which the virus in nonproducer cells either lacks or does not express.