Needle in a Whey-Stack: PhRACS as a Discovery Tool for Unknown Phage-Host Combinations-Reference-Cited by-同舟云学术

Needle in a Whey-Stack: PhRACS as a Discovery Tool for Unknown Phage-Host Combinations

Published:2022-02-22 Issue:1 Volume:13 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Casey Eoghan¹²,McDonnell Brian¹^ORCID,White Kelsey¹²,Stamou Panagiota²³,Crowley Tadhg²³,O’Neill Ian¹²,Lavelle Katherine¹²^ORCID,Hayes Stephen¹,Lugli Gabriele A.⁴,Arboleya Silvia⁵,James Kieran²^ORCID,Ventura Marco⁴^ORCID,Martinez Ines⁶,Gueimonde Miguel⁵,dal Bello Fabio⁶,Nally Ken²³,Mahony Jennifer¹²,van Sinderen Douwe¹²^ORCID

Affiliation:

1. School of Microbiology, University College Cork, Cork, Ireland

2. APC Microbiome Ireland, University College Cork, Cork, Ireland

3. Flow Cytometry Platform, APC Microbiome Ireland, University College Cork, Cork, Ireland

4. Laboratory of Probiogenomics, Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy

5. Microbiota, Food and Health Group, Department of Biochemistry and Microbiology of Dairy Products, Institutode Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas, Villaviciosa, Spain

6. Sacco Srl, Cadorago (Co), Italy

Abstract

PhRACS aims to bridge the current divide betweenin silicogenetic analyses (i.e., phageomic studies) and traditional culture-based methodology. Through the labeling of specific bacterial hosts with fluorescently tagged recombinant phage receptor binding proteins and the isolation of tagged cells using flow cytometry, PhRACS allows the full potential of phageomic data to be realized in the wet laboratory.

Funder

Joint Programming Initiative A healthy diet for a healthy life

Science Foundation Ireland

Ministry of Science, Innovation and Universities

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mbio.03334-21

Reference67 articles.

1. An improved plaque assay for poor plaque-producing temperate lactococcal bacteriophages