Affiliation:
1. Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland 21201
Abstract
ABSTRACT
Based upon the lipopolysaccharide (LPS) structure and antigenicity of
Shigella
group B, a strategy for broad cross-protection against 14
Shigella flexneri
serotypes was designed. This strategy involves the use of two
S. flexneri
serotypes (2a and 3a), which together bear the all of the major antigenic group factors of this group. The novel attenuated strains used in these studies were
S. flexneri
2a strain CVD 1207 (Δ
guaB-A ΔvirG Δset1 Δsen
) and
S. flexneri
3a strain CVD 1211 (Δ
guaB-A ΔvirG Δsen
). Guinea pigs were immunized with an equal mixture of these strains and later challenged (Sereny test) with a wild-type
S. flexneri
serotype 1a, 1b, 2b, 4b, 5b, Y, or 6 strain of demonstrated virulence in the same model. Guinea pigs that were immunized with these two vaccine strains produced serum and mucosal antibodies that cross-reacted with all the
S. flexneri
serotypes tested (except of
S. flexneri
serotype 6) as assessed by enzyme-linked immunosorbent assay, immunoblotting, and slide agglutination. Furthermore, the combination vaccine conferred significant protection against challenge with
S. flexneri
serotypes 1b, 2b, 5b, and Y but not with serotypes 1a, 4b, or (as predicted) 6.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
127 articles.
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