Affiliation:
1. Department of Botany
2. Department of Chemical Engineering, University of Toronto, Toronto, Ontario, Canada
Abstract
ABSTRACT
Dehalobacter
and “
Dehalococcoides
” spp. were previously shown to be involved in the biotransformation of 1,1,2-trichloroethane (1,1,2-TCA) and 1,2-dichloroethane (1,2-DCA) to ethene in a mixed anaerobic enrichment culture. Here we report the further enrichment and characterization of a
Dehalobacter
sp. from this mixed culture in coculture with an
Acetobacterium
sp. Through a series of serial transfers and dilutions with acetate, H
2
, and 1,2-DCA, a stable coculture of
Acetobacterium
and
Dehalobacter
spp. was obtained, where
Dehalobacter
grew during dechlorination. The isolated
Acetobacterium
strain did not dechlorinate 1,2-DCA. Quantitative PCR with specific primers showed that
Dehalobacter
cells did not grow in the absence of a chlorinated electron acceptor and that the growth yield with 1,2-DCA was 6.9 (±0.7) × 10
7
16S rRNA gene copies/μmol 1,2-DCA degraded. PCR with degenerate primers targeting reductive dehalogenase genes detected three distinct
Dehalobacter
/
Desulfitobacterium
-type sequences in the mixed-parent culture, but only one of these was present in the 1,2-DCA-H
2
coculture. Reverse transcriptase PCR revealed the transcription of this dehalogenase gene specifically during the dechlorination of 1,2-DCA. The 1,2-DCA-H
2
coculture could dechlorinate 1,2-DCA but not 1,1,2-TCA, nor could it dechlorinate chlorinated ethenes. As a collective, the genus
Dehalobacter
has been show to dechlorinate many diverse compounds, but individual species seem to each have a narrow substrate range.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
79 articles.
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