Scaffoldin Modules Serving as “Cargo” Domains To Promote the Secretion of Heterologous Cellulosomal Cellulases by Clostridium acetobutylicum-Reference-Cited by-同舟云学术

Scaffoldin Modules Serving as “Cargo” Domains To Promote the Secretion of Heterologous Cellulosomal Cellulases by Clostridium acetobutylicum

Published:2011-09 Issue:17 Volume:77 Page:6277-6280
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Chanal Angélique¹²,Mingardon Florence¹,Bauzan Marielle³,Tardif Chantal¹⁴,Fierobe Henri-Pierre¹

Affiliation:

1. Laboratoire de Chimie Bactérienne CNRS IMM, 31 Chemin Joseph Aiguier, 13402 Marseille, France

2. Total Gas and Power, R & D Biotechnology Team, 92078 Paris-La Défense 6, France

3. Unité de Fermentation et Cultures de Microorganismes, CNRS IMM 31, Chemin Joseph Aiguier, 13402 Marseille, France

4. Université de Provence, 3 Pl. Victor Hugo, 13331 Marseille, France

Abstract

ABSTRACT The secretion of large heterologous cellulases by Clostridium acetobutylicum was formerly shown to be deleterious. To circumvent this issue, various scaffoldins' modules were grafted at their N termini. Family 3a cellulose binding module combined with an X2 module(s) was found to trigger the secretion of Clostridium cellulolyticum cellulases by the solventogenic bacterium.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00758-11

Reference17 articles.

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