Nitrogenase Switch-Off by Ammonium Ions in Azospirillum brasilense Requires the GlnB Nitrogen Signal-Transducing Protein-Reference-Cited by-同舟云学术

Nitrogenase Switch-Off by Ammonium Ions in Azospirillum brasilense Requires the GlnB Nitrogen Signal-Transducing Protein

Published:2005-09 Issue:9 Volume:71 Page:5637-5641
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Klassen Giseli¹,Souza Emanuel M.²,Yates M. Geoffrey²,Rigo Liu Un²,Costa Roberta M.¹,Inaba Juliana²,Pedrosa Fábio O.²

Affiliation:

1. Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, Brasil

2. Departamento de Bioquímica e Biologia Molecular

Abstract

ABSTRACT Nitrogenase activity in several diazotrophs is switched off by ammonium and reactivated after consumption. The signaling pathway to this system in Azospirillum brasilense is not understood. We show that ammonium-dependent switch-off through ADP-ribosylation of Fe protein was partial in a glnB mutant of A. brasilense but absent in a glnB glnZ double mutant. Triggering of inactivation by anaerobic conditions was not affected in either mutant. The results suggest that glnB is necessary for full ammonium-dependent nitrogenase switch-off in A. brasilense .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.9.5637-5641.2005

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