Isolation and Characterization of Temperate Bacteriophages of Clostridium difficile

Author:

Goh Shan1,Riley Thomas V.12,Chang Barbara J.1

Affiliation:

1. Microbiology, School of Biomedical and Chemical Sciences, Queen Elizabeth II Medical Centre, The University of Western Australia

2. Division of Microbiology and Infectious Diseases, The Western Australian Centre for Pathology and Medical Research, Nedlands, Western Australia, Australia

Abstract

ABSTRACT The lack of information on bacteriophages of Clostridium difficile prompted this study. Three of 56 clinical C. difficile isolates yielded double-stranded DNA phages φC2, φC5, φC6, and φC8 upon induction. Superinfection and DNA analyses revealed relatedness between the phages, while partial sequencing of φC2 showed nucleotide homology to the sequenced C. difficile strain CD630.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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