Enzyme-linked immunosorbent assay for serodiagnosis of Mycoplasma pneumoniae infections

Author:

Busolo F,Tonin E,Meloni G A

Abstract

Enzyme-linked immunosorbent assay (ELISA) was used for detection of antibodies against Mycoplasma pneumoniae. The results of ELISA were compared with those of other serological methods, such as complement fixation, metabolic inhibition, mycoplasmacidal test, and radioimmunoprecipitation. ELISA and mycoplasmacidal test showed greater sensitivity than did complement fixation and metabolic inhibition. Radioimmunoprecipitation showed serum titers 2- to 16-fold higher. Specificity of ELISA was determined by testing other human mycoplasmas. A simplified method based on the determination of ELISA antibody end-point titer by a single serum dilution is proposed.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference11 articles.

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2. Comparative evaluation of some methods for detection of Mycoplasma pneumoniae antibodies;Busolo F.;Boll. Ist. Sieroter. Milan.,1979

3. Clyde W. A. Jr. 1979. Mycoplasma pneumoniae infections of man p. 275-306. In J. G. Tully and R. F. Whitcomb (ed.) Human and animal mycoplasmas vol. III. Academic Press Inc. New York.

4. Enzyme-linked immunosorbent assay (ELISA). III. Quantitation of specific antibodies by enzyme labeled anti-immunoglobuline in antigen-coated tubes;Engvall E.;J. Immunol.,1972

5. Antigen requirements, sensitivity, and specificity of enzyme immunoassays for measles and rubella viral antibodies;Forghani B.;J. Clin. Microbiol.,1979

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