Symbiont Succession during Embryonic Development of the European Medicinal Leech, Hirudo verbana

Author:

Rio Rita V. M.1,Maltz Michele2,McCormick Benjamin1,Reiss Alexander2,Graf Joerg2

Affiliation:

1. Department of Biology, West Virginia University, Morgantown, West Virginia 26506

2. Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269

Abstract

ABSTRACT The European medicinal leech, Hirudo verbana , harbors simple microbial communities in the digestive tract and bladder. The colonization history, infection frequency, and growth dynamics of symbionts through host embryogenesis are described using diagnostic PCR and quantitative PCR. Symbiont species displayed diversity in temporal establishment and proliferation through leech development.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference18 articles.

1. Busing, K.-H. 1951. Pseudomonas hirudinis, ein bakterieller Darmsymbiont des Blutegels (Hirudo officinalis). Zentralbl. Bakteriol.157:478-485.

2. Busing, K.-H., W. Doll, and K. Freytag. 1953. Die Bakterienflora der medizinischen Blutegel. Arch. Mikrobiol.19:52-86.

3. Fernandez, J., and G. S. Stent. 1982. Embryonic development of the hirudinid leech Hirudo medicinalis: structure, development and segmentation of the germinal plate. J. Embryol. Exp. Morphol.72:71-96.

4. Graf, J. 2002. The effects of symbionts on the physiology of Hirudo medicinalis, the medicinal leech. Invert. Reprod. Dev.41:269-275.

5. Graf, J., Y. Kikuchi, and R. V. Rio. 2006. Leeches and their microbiota: naturally simple symbiosis models. Trends Microbiol.14:365-371.

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