Affiliation:
1. Department of Microbiology and Immunology, University of Leicester, Leicester LE1 9HN, United Kingdom
Abstract
ABSTRACT
The activity of H
2
O
2
against the resistant cyst stage of the pathogenic free-living amoeba
Acanthamoeba
was enhanced by the addition of KI and either horseradish peroxidase or soybean peroxidase or, to a lesser degree, lactoperoxidase. This resulted in an increase in the cysticidal activity of 3% (wt/vol) H
2
O
2
, and there was >3-log killing in 2 h, compared with the 6 h required for comparable results with the peroxide solution alone (
P
< 0.05). With 2% H
2
O
2
, enhancement was observed at all time points (
P
< 0.05), and total killing of the cyst inoculum occurred at 4 h, compared with 6 h for the peroxide alone. The activity of sublethal 1% H
2
O
2
was enhanced to give 3-log killing after 8 h of exposure (
P
< 0.05). No enhancement was obtained when KCl or catalase was used as a substitute in the reaction mixtures. The H
2
O
2
was not neutralized in the enhanced system during the experiments. However, in the presence of a platinum disk used to neutralize H
2
O
2
in contact lens care systems, the enhanced 2% H
2
O
2
system gave 2.8-log killing after 6 h or total cyst killing by 8 h, and total neutralization of the H
2
O
2
occurred by 4 h. In contrast, 2% H
2
O
2
alone resulted in <0.8-log killing of cysts in the presence of the platinum disk due to rapid (<1 h) neutralization of the peroxide. Our observations could result in significant improvement in the efficacy of H
2
O
2
contact lens disinfection systems against
Acanthamoeba
cysts and prevention of acanthamoeba keratitis.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference28 articles.
1. Agner K. 1972. Biological effects of hyperchlorous acid formed by “MPO” peroxidation in the presence of chloride ions p. 329-335. In A. Akeson and A. Ehrenberg (ed.) Structure and function of oxidation-reduction enzymes vol. 18. Pergamon Press New York N.Y.
2. Allen R. C. September 1995. Method for killing yeast and sporular microorganisms with haloperoxidase-containing compositions. U.S. patent 5 451 402.
3. Allen R. C. February 1995. Halo-peroxidase containing compositions for killing yeast and sporular microorganisms. U.S. patent 5 389 369.
4. A quantitative method to evaluate neutralizer toxicity against Acanthamoeba castellanii
5. Gardener J. F. and M. M. Peel. 1986. Introduction to sterilization and disinfection. Churchill Livingstone London United Kingdom.
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