Ecological Observations Based on Functional Gene Sequencing Are Sensitive to the Amplicon Processing Method

Author:

Cholet Fabien1ORCID,Lisik Agata2,Agogué Hélène3,Ijaz Umer Z.1,Pineau Philippe3,Lachaussée Nicolas3,Smith Cindy J.1ORCID

Affiliation:

1. Infrastructure and Environment, James Watt School of Engineering, University of Glasgow, Glasgow, Scotland

2. Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland

3. Université de La Rochelle, CNRS, UMR 7266, LIENSs, La Rochelle, France

Abstract

Several analysis pipelines are available to microbial ecologists to process amplicon sequencing data, yet to date, there is no consensus as to the most appropriate method, and it becomes more difficult for genes that encode a specific function (functional genes). Standardized approaches need to be adopted to increase the reliability and reproducibility of environmental amplicon-sequencing-based data sets.

Funder

Environmental Protection Agency

UKRI | Natural Environment Research Council

Royal Academy of Engineering

UKRI | Engineering and Physical Sciences Research Council

Thomas Crawford Hayes NUIG

Harry Smith Vacation Studenship

Mobility Grant French Enbassy in Ireland

Microbiology Society Research Visit Grant

University of Glasgow | James Watt School of Engineering, University of Glasgow

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3