Intracellular Phage Tail-Like Nanostructures Affect Susceptibility of Streptomyces lividans to Osmotic Stress-Reference-Cited by-同舟云学术

Intracellular Phage Tail-Like Nanostructures Affect Susceptibility of Streptomyces lividans to Osmotic Stress

Published:2023-06-22 Issue:3 Volume:8 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Nagakubo Toshiki¹^ORCID,Asamizu Shumpei¹²^ORCID,Yamamoto Tatsuya³,Kato Manami¹,Nishiyama Tatsuya⁴,Toyofuku Masanori³⁵,Nomura Nobuhiko³⁵,Onaka Hiroyasu¹²

Affiliation:

1. Graduate School of Agricultural and Life Sciences, Department of Biotechnology, The University of Tokyo, Tokyo, Japan

2. Collaborative Research Institute for Innovative Microbiology (CRIIM), The University of Tokyo, Tokyo, Japan

3. Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan

4. Life Science Research Center, College of Bioresource Sciences, Nihon University, Tokyo, Japan

5. Microbiology Research Center for Sustainability (MiCS), University of Tsukuba, Tsukuba, Japan

Abstract

Recent bioinformatic analyses have revealed that CIS-related gene clusters are highly conserved in Gram-positive actinomycetes, especially members of the genus Streptomyces known for their ability to produce therapeutic antibiotics. While typical CISs are released from the cells and can act as protein translocation systems that inject effector proteins into the target cells, our results indicate the unique intracellular localization of SLPs, CIS-related nanostructures produced by S. lividans . In addition, the direct and indirect interactions of SLPs with cytoplasmic proteins and SLP localization within specific regions of mycelia suggest that the biological significance of SLPs is related to intracellular processes.

Funder

Japan Society for the Promotion of Science

Amano Enzyme Inc.

Japan Science and Technology Agency

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/msphere.00114-23

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