Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing-Reference-Cited by-同舟云学术

Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing

Published:2022-06-29 Issue:3 Volume:7 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Thurlow Charles M.¹^ORCID,Joseph Sandeep J.¹^ORCID,Ganova-Raeva Lilia²,Katz Samantha S.¹,Pereira Lara¹,Chen Cheng¹,Debra Alyssa¹,Vilfort Kendra¹,Workowski Kimberly¹³,Cohen Stephanie E.⁴,Reno Hilary⁵⁶,Sun Yongcheng¹,Burroughs Mark⁷,Sheth Mili⁷,Chi Kai-Hua¹,Danavall Damien¹,Philip Susan S.³,Cao Weiping¹,Kersh Ellen N.¹^ORCID,Pillay Allan¹^ORCID

Affiliation:

1. Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

2. Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

3. Department of Medicine, Emory University, Atlanta, Georgia, USA

4. San Francisco Department of Public Health, San Francisco, California, USA

5. St. Louis County Sexual Health Clinic, St. Louis, Missouri, USA

6. Division of Infectious Diseases, Washington University, St. Louis, Missouri, USA

7. Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Abstract

Syphilis is a sexually transmitted, disseminated acute and chronic infection caused by the bacterial pathogen Treponema pallidum subspecies pallidum . Primary syphilis typically presents as single or multiple mucocutaneous lesions and, if left untreated, can progress through multiple stages with various clinical manifestations.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/msphere.00009-22

Reference47 articles.

1. CDC. 2020. Sexually transmitted disease surveillance 2019. US Department of Health and Human Services, Atlanta, GA.

2. Macrolide Resistance inTreponema pallidumin the United States and Ireland

3. Syphilis testing, typing, and treatment follow-up

4. Macrolide Resistance in the Syphilis Spirochete, Treponema pallidum ssp. pallidum: Can We Also Expect Macrolide-Resistant Yaws Strains?

5. Detection of the A2058G and A2059G 23S rRNA Gene Point Mutations Associated with Azithromycin Resistance in Treponema pallidum by Use of a TaqMan Real-Time Multiplex PCR Assay

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