Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing-Reference-Cited by-同舟云学术

Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing

Published:2022-06-29 Issue:3 Volume:7 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Thurlow Charles M.¹^ORCID,Joseph Sandeep J.¹^ORCID,Ganova-Raeva Lilia²,Katz Samantha S.¹,Pereira Lara¹,Chen Cheng¹,Debra Alyssa¹,Vilfort Kendra¹,Workowski Kimberly¹³,Cohen Stephanie E.⁴,Reno Hilary⁵⁶,Sun Yongcheng¹,Burroughs Mark⁷,Sheth Mili⁷,Chi Kai-Hua¹,Danavall Damien¹,Philip Susan S.³,Cao Weiping¹,Kersh Ellen N.¹^ORCID,Pillay Allan¹^ORCID

Affiliation:

1. Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

2. Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

3. Department of Medicine, Emory University, Atlanta, Georgia, USA

4. San Francisco Department of Public Health, San Francisco, California, USA

5. St. Louis County Sexual Health Clinic, St. Louis, Missouri, USA

6. Division of Infectious Diseases, Washington University, St. Louis, Missouri, USA

7. Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Abstract

Syphilis is a sexually transmitted, disseminated acute and chronic infection caused by the bacterial pathogen Treponema pallidum subspecies pallidum . Primary syphilis typically presents as single or multiple mucocutaneous lesions and, if left untreated, can progress through multiple stages with various clinical manifestations.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/msphere.00009-22

Reference47 articles.

1. CDC. 2020. Sexually transmitted disease surveillance 2019. US Department of Health and Human Services, Atlanta, GA.

2. Macrolide Resistance inTreponema pallidumin the United States and Ireland

3. Syphilis testing, typing, and treatment follow-up

4. Macrolide Resistance in the Syphilis Spirochete, Treponema pallidum ssp. pallidum: Can We Also Expect Macrolide-Resistant Yaws Strains?

5. Detection of the A2058G and A2059G 23S rRNA Gene Point Mutations Associated with Azithromycin Resistance in Treponema pallidum by Use of a TaqMan Real-Time Multiplex PCR Assay

Cited by 18 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Turning the needle into the haystack: Culture-independent amplification of complex microbial genomes directly from their native environment;PLOS Pathogens;2024-09-12

2. Advances in Host Depletion and Pathogen Enrichment Methods for Rapid Sequencing–Based Diagnosis of Bloodstream Infection;The Journal of Molecular Diagnostics;2024-09

3. Molecular investigation of Treponema pallidum strains associated with ocular syphilis in the United States, 2016–2020;Microbiology Spectrum;2024-08-20

4. Treponema pallidum genetic diversity and its implications for targeted vaccine development: A cross-sectional study of early syphilis cases in Southwestern Colombia;PLOS ONE;2024-07-19

5. Contribution of the patient microbiome to surgical site infection and antibiotic prophylaxis failure in spine surgery;Science Translational Medicine;2024-04-10