Genetic Characterization of Plasmid-Borne bla OXA-58 in Distinct Acinetobacter Species

Author:

Matos Adriana P.1,Cayô Rodrigo12,Almeida Luiz G. P.3,Streling Ana Paula1,Nodari Carolina S.1,Martins Willames M. B. S.1,Narciso Ana Clara1,Silva Rosa M.4,Vasconcelos Ana T. R.3,Gales Ana C.1

Affiliation:

1. Universidade Federal de São Paulo—UNIFESP, Laboratório Alerta, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina—EPM, São Paulo, São Paulo, Brazil

2. Universidade Federal de São Paulo—UNIFESP, Laboratório de Imunologia e Bacteriologia—LIB, Setor de Biologia Molecular, Microbiologia e Imunologia—Departamento de Ciências Biológicas—CDB, Instituto de Ciências Ambientais, Químicas e Farmacêuticas—ICAQF, Diadema, São Paulo, Brazil

3. National Laboratory for Scientific Computing—LNCC, Petrópolis, Rio de Janeiro, Brazil

4. Universidade Federal de São Paulo—UNIFESP, Laboratório de Microbiologia Básica, Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina—EPM, São Paulo, São Paulo, Brazil

Abstract

Although the bla OXA-58 gene has been infrequently described in Brazil, contrasting with other bordering South American countries, we verified the maintenance of this resistance determinant over time among carbapenem-resistant Acinetobacter species isolates, not only in nosocomial settings but also in the environment. In addition, to the best of our knowledge, this is the first study to have used WPS analysis to evaluate the genetic surroundings of bla OXA-58 in Brazil. Moreover, the A. seifertii and A. baumannii clinical strains evaluated in this study were recovered 17 years apart in hospitals located in distinct Brazilian geographic regions.

Funder

MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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