Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics-Reference-Cited by-同舟云学术

Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics

Published:2021-10-27 Issue:5 Volume:6 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Guan Huihui¹,Pu Yanni¹,Liu Chenglin¹,Lou Tao¹²,Tan Shishang¹²,Kong Mengmeng¹,Sun Zhonghan¹,Mei Zhendong¹,Qi Qibin³,Quan Zhexue⁴^ORCID,Zhao Guoping⁵,Zheng Yan⁶⁷⁸^ORCID

Affiliation:

1. Human Phenome Institute, School of Life Sciences, Fudan University, Shanghai, China

2. MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China

3. Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, New York, USA

4. Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, School of Life Sciences, Fudan University, Shanghai, China

5. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

6. State Key Laboratory of Genetic Engineering School of Life Sciences, Human Phenome Institute, Fudan University, Shanghai, China

7. Ministry of Education Key Laboratory of Public Health Safety, School of Public Health, Fudan University, Shanghai, China

8. Yiwu Research Institute of Fudan University, Shanghai, China

Abstract

The choice of fecal collection method is essential for studying gut microbe-human interactions in large-scale population-based research. In this study, we examined the effects of fecal collection methods and storage time at ambient temperature on variations in the gut microbiome community composition; microbial diversity metrics at the species, gene, and pathway levels; antibiotic resistance genes; and metabolome profiling.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSphere.00636-21

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