The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis-Reference-Cited by-同舟云学术

The Human Sodium Iodide Symporter as a Reporter Gene for Studying Middle East Respiratory Syndrome Coronavirus Pathogenesis

Published:2018-12-26 Issue:6 Volume:3 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Chefer Svetlana¹,Seidel Jurgen¹,Cockrell Adam S.²,Yount Boyd²,Solomon Jeffrey³,Hagen Katie R.¹,Liu David X.¹,Huzella Louis M.¹,Kumar Mia R.⁴,Postnikova Elena¹,Bohannon J. Kyle¹,Lackemeyer Matthew G.¹,Cooper Kurt¹,Endlich-Frazier Ariel⁴,Sharma Heema⁴,Thomasson David¹,Bartos Christopher¹,Sayre Philip J.¹,Sims Amy²,Dyall Julie¹,Holbrook Michael R.¹,Jahrling Peter B.¹⁴,Baric Ralph S.²,Johnson Reed F.⁴

Affiliation:

1. Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA

2. Department of Epidemiology, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA

3. Clinical Research Directorate/Clinical Monitoring Research Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA

4. Emerging Viral Pathogens Section, Laboratory of Immunoregulation, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA

Abstract

Advanced medical imaging such as single photon emission computed tomography with computed tomography (SPECT/CT) enhances fields such as oncology and cardiology. Application of SPECT/CT, magnetic resonance imaging, and positron emission tomography to infectious disease may enhance pathogenesis studies and provide alternate biomarkers of disease progression. The experiments described in this article focus on insertion of a SPECT/CT-compatible reporter gene into MERS-CoV to demonstrate that a functional SPECT/CT reporter gene can be inserted into a virus.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

Battelle

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSphere.00540-18

Reference34 articles.

1. A novel Ebola virus expressing luciferase allows for rapid and quantitative testing of antivirals