The Lumen of Human Intestinal Organoids Poses Greater Stress to Bacteria Compared to the Germ-Free Mouse Intestine: Escherichia coli Deficient in RpoS as a Colonization Probe-Reference-Cited by-同舟云学术

The Lumen of Human Intestinal Organoids Poses Greater Stress to Bacteria Compared to the Germ-Free Mouse Intestine: Escherichia coli Deficient in RpoS as a Colonization Probe

Published:2020-12-23 Issue:6 Volume:5 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Barron Madeline R.¹^ORCID,Cieza Roberto J.²^ORCID,Hill David R.³,Huang Sha³,Yadagiri Veda K.²,Spence Jason R.³⁴⁵^ORCID,Young Vincent B.¹²^ORCID

Affiliation:

1. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA

2. Department of Internal Medicine, Division of Infectious Diseases, University of Michigan Medical School, Ann Arbor, Michigan, USA

3. Department of Internal Medicine, Division of Gastroenterology, University of Michigan Medical School, Ann Arbor, Michigan, USA

4. Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan, USA

5. Department of Biomedical Engineering, University of Michigan Medical School, Ann Arbor, Michigan, USA

Abstract

Technological advancements have driven and will continue to drive the adoption of organotypic systems for investigating host-microbe interactions within the human intestinal ecosystem. Using E. coli deficient in the RpoS-mediated general stress response, we demonstrate that the type or severity of microbial stressors within the HIO lumen is more restrictive than those of the in vivo environment of the germ-free mouse gut. This study provides important insight into the nature of the HIO microenvironment from a microbiological standpoint.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSphere.00777-20

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