Whole-Genome Enrichment and Sequencing of Chlamydia trachomatis Directly from Patient Clinical Vaginal and Rectal Swabs-Reference-Cited by-同舟云学术

Whole-Genome Enrichment and Sequencing of Chlamydia trachomatis Directly from Patient Clinical Vaginal and Rectal Swabs

Published:2021-04-28 Issue:2 Volume:6 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Bowden Katherine E.¹^ORCID,Joseph Sandeep J.¹,Cartee John C.¹,Ziklo Noa²,Danavall Damien¹,Raphael Brian H.¹,Read Timothy D.³^ORCID,Dean Deborah⁴²^ORCID

Affiliation:

1. Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

2. University of California San Francisco Benioff Children’s Hospital Oakland Research Institute, Oakland, California, USA

3. Emory University School of Medicine, Atlanta, Georgia, USA

4. University of California San Francisco School of Medicine, San Francisco, California, USA

Abstract

Chlamydia trachomatis is an obligate intracellular bacterium that is not easily cultured, which limits our understanding of urogenital and rectal C. trachomatis transmission and impact on morbidity. To provide a publicly available workflow for whole-genome target enrichment and sequencing of C. trachomatis directly from clinical urine, vaginal, conjunctival, and rectal specimens, we developed and report on an RNA bait library to enrich the organism from clinical samples for sequencing.

Funder

Office of Advanced Molecular Detection at CDC

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSphere.01302-20

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