k -mer-Based Metagenomics Tools Provide a Fast and Sensitive Approach for the Detection of Viral Contaminants in Biopharmaceutical and Vaccine Manufacturing Applications Using Next-Generation Sequencing-Reference-Cited by-同舟云学术

k -mer-Based Metagenomics Tools Provide a Fast and Sensitive Approach for the Detection of Viral Contaminants in Biopharmaceutical and Vaccine Manufacturing Applications Using Next-Generation Sequencing

Published:2021-04-28 Issue:2 Volume:6 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

MacDonald Madolyn L.¹²^ORCID,Polson Shawn W.²³⁴^ORCID,Lee Kelvin H.¹²^ORCID

Affiliation:

1. Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, USA

2. Ammon Pinizzotto Biopharmaceutical Innovation Center, Newark, Delaware, USA

3. Center for Bioinformatics and Computational Biology, University of Delaware, Newark, Delaware, USA

4. Department of Computer and Information Sciences, University of Delaware, Newark, Delaware, USA

Abstract

Next-generation sequencing (NGS) has been proposed as a complementary method to detect adventitious viruses in the production of biotherapeutics and vaccines to current in vivo and in vitro methods. Before NGS can be established in industry as a main viral detection technology, further investigation into the various aspects of bioinformatics analyses required to identify and classify viral NGS reads is needed.

Funder

DOC | National Institute of Standards and Technology

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSphere.01336-20

Reference60 articles.

1. Viral contamination in biologic manufacture and implications for emerging therapies

2. Experience with viral contamination in cell culture;Garnick RL;Dev Biol Stand,1996