Antigenic Characterization of New Lineage II Insect-Specific Flaviviruses in Australian Mosquitoes and Identification of Host Restriction Factors

Author:

Harrison Jessica J.12ORCID,Hobson-Peters Jody12,Colmant Agathe M. G.12,Koh Joanna12,Newton Natalee D.12,Warrilow David3,Bielefeldt-Ohmann Helle12,Piyasena Thisun B. H.12,O’Brien Caitlin A.12,Vet Laura J.12,Paramitha Devina12,Potter James R.12,Davis Steven S.4,Johansen Cheryl A.56,Setoh Yin Xiang12,Khromykh Alexander A.12,Hall Roy A.12

Affiliation:

1. School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Queensland, Australia

2. Australian Infectious Diseases Research Centre, The University of Queensland, St. Lucia, Queensland, Australia

3. Public Health Virology Laboratory, Department of Health, Queensland Government, Archerfield, Queensland, Australia

4. Berrimah Veterinary Laboratory, Department of Primary Industries and Fisheries, Darwin, Australia

5. School of Pathology and Laboratory Medicine, WA, Nedlands, Western Australia, Australia

6. PathWest Laboratory Medicine WA, Nedlands, Western Australia, Australia

Abstract

The globally important flavivirus pathogens West Nile virus, Zika virus, dengue viruses, and yellow fever virus can infect mosquito vectors and be transmitted to humans and other vertebrate species in which they cause significant levels of disease and mortality. However, the subgroup of closely related flaviviruses, known as lineage II insect-specific flaviviruses (Lin II ISFs), only infect mosquitoes and cannot replicate in cells of vertebrate origin. Our data are the first to uncover the mechanisms that restrict the growth of Lin II ISFs in vertebrate cells and provides new insights into the evolution of these viruses and the mechanisms associated with host switching that may allow new mosquito-borne viral diseases to emerge. The new reagents generated in this study, including the first Lin II ISF-reactive monoclonal antibodies and Lin II ISF mutants and chimeric viruses, also provide new tools and approaches to enable further research advances in this field.

Funder

Department of Health | National Health and Medical Research Council

Department of Education and Training | Australian Research Council

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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