Circular Whole-Transcriptome Amplification (cWTA) and mNGS Screening Enhanced by a Group Testing Algorithm (mEGA) Enable High-Throughput and Comprehensive Virus Identification-Reference-Cited by-同舟云学术

Circular Whole-Transcriptome Amplification (cWTA) and mNGS Screening Enhanced by a Group Testing Algorithm (mEGA) Enable High-Throughput and Comprehensive Virus Identification

Published:2022-10-26 Issue:5 Volume:7 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Reteng Patrick¹,Nguyen Thuy Linh²,Rahman Mizanur³^ORCID,Bispo de Filippis Ana Maria⁴,Hayashida Kyoko¹⁵,Sugi Tatsuki¹⁵,Gonzalez Gabriel⁶,Hall William W.⁷⁸,Nguyen Thi Lan Anh²,Yamagishi Junya¹⁵^ORCID

Affiliation:

1. Division of Collaboration and Education, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan

2. Laboratory for Molecular Diagnostics, National Institute of Epidemiology and Health, Hanoi, Vietnam

3. Evercare Hospital Dhaka, Dhaka, Bangladesh

4. Flavivirus Laboratory, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil

5. International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan

6. National Virus Reference Laboratory, University College Dublin, Dublin, Ireland

7. School of Medicine and Medical Research, University College Dublin, Dublin, Ireland

8. Global Virus Network, Baltimore, Maryland, USA

Abstract

Given the breadth of pathogens that cause infections, a single approach that can detect a wide range of pathogens is ideal but is impractical due to the available tests being highly specific to a certain pathogen. Recent developments in sequencing technology have introduced mNGS as an alternative that provides detection of a wide-range of pathogens by detecting the presence of their nucleic acids in the sample.

Funder

MEXT | Japan Society for the Promotion of Science

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/msphere.00332-22

Reference37 articles.

1. Diversity of infectious aetiologies of acute undifferentiated febrile illnesses in south and Southeast Asia: a systematic review

2. Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

3. Fever and Fever of Unknown Origin: Review, Recent Advances, and Lingering Dogma

4. Clinical metagenomics

5. A Novel Pan-FlavivirusDetection and Identification Assay Based on RT-qPCR and Microarray