Development and Evaluation of a Novel VHH-Based Immunocapture Assay for High-Sensitivity Detection of Shiga Toxin Type 2 (Stx2) in Stool Samples-Reference-Cited by-同舟云学术

Development and Evaluation of a Novel VHH-Based Immunocapture Assay for High-Sensitivity Detection of Shiga Toxin Type 2 (Stx2) in Stool Samples

Published:2020-02-24 Issue:3 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Melli Luciano J.¹,Zylberman Vanesa²,Hiriart Yanina²,Lauche Constanza E.²,Baschkier Ariela³,Pardo Romina²,Miliwebsky Elizabeth³,Chinen Isabel³,Rivas Marta³,Goldbaum Fernando A.²,Ugalde Juan E.¹,Comerci Diego J.¹⁴,Ciocchini Andrés E.¹

Affiliation:

1. Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde, Universidad Nacional de San Martín (IIB-UNSAM-CONICET), San Martín, Buenos Aires, Argentina

2. Inmunova S.A., San Martín, Buenos Aires, Argentina

3. Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas-ANLIS Dr. Carlos G. Malbrán, Ciudad Autónoma de Buenos Aires, Argentina

4. Comisión Nacional de Energía Atómica, Grupo Pecuario, Centro Atómico Ezeiza, Buenos Aires, Argentina

Abstract

Shiga toxin (Stx)-producing Escherichia coli (STEC) is the main cause of postdiarrheal hemolytic-uremic syndrome (HUS), a life-threatening clinical complication characterized by hemolytic anemia, thrombocytopenia, and acute renal failure that mainly affects children. A relevant feature of STEC strains is the production of Stx, and all of them express Stx1 and/or Stx2 regardless of the strain serotype.

Funder

MINCyT | Agencia Nacional de Promoción Científica y Tecnológica

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01566-19

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