Affiliation:
1. Department of Microbiology & Cell Biology, Indian Institute of Science, Bangalore, India
Abstract
Rotavirus is known to replicate in specialized virus-induced cytoplasmic inclusion bodies called viroplasms (VMs), but the composition and structure of VMs are not yet understood. Here we demonstrate that rotavirus interferes with normal SG and PB assembly but promotes formation of atypical SG-PB structures by selective exclusion of a few components and employs a novel strategy of sequestration of the remodeled SG-PB granules in the VMs to promote virus growth by modulating their negative influence on virus infection. Rotavirus VMs appear to be complex supramolecular structures formed by the union of the triad of viral replication complexes and remodeled SGs and PBs, as well as other host factors, and designed to promote productive virus infection. These observations have implications for the planning of future research with the aim of understanding the structure of the VM, the mechanism of morphogenesis of the virus, and the detailed roles of host proteins in rotavirus biology.
Funder
Department of Science and Technology, Ministry of Science and Technology
Department of Biotechnology , Ministry of Science and Technology
Indian National Science Academy
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference136 articles.
1. Estes MK, Greenberg HB. 2013. Rotaviruses, p 1347−1401. In Knipe DM, Howley PM, Cohen JI, Griffin DE, Lamb RA, Martin MA, Racaniello VR, Roizman B (ed), Fields virology, 6th ed. Lippincott Williams & Wilkins, Philadelphia, PA.
2. Coding assignments of double-stranded RNA segments of SA 11 rotavirus established by in vitro translation
3. Three-dimensional structure of rotavirus
4. Atomic model of an infectious rotavirus particle
5. Rotavirus gene structure and function
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