Author:
Lugowski C,Kułakowska M,Romanowska E
Abstract
The methods of limited periodate oxidation and reductive amination were used to obtain covalently linked enterobacterial common antigen (ECA) with tetanus toxoid. This procedure is simple and gives a good yield of the conjugate with high ECA content (molecular ratio of ECA to tetanus toxoid, 6:1). The ECA-tetanus toxoid conjugate is immunogenic in rabbits, in contrast to free ECA or a mixture of ECA with proteins. This conjugate produces high levels of ECA-specific immunoglobulin G antibodies, which can be used as a standard serum.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference38 articles.
1. Chemo-immunological studies on conjugated carbohydrate-proteins. V. The immunological specificity of an antigen prepared by combining the capsular polysaccharide III pneumococcus with foreign protein;Avery 0.;J. Exp. Med.,1931
2. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents;Avrameas S.;Immunochemistry,1969
3. Meningococcal group C polysaccharide etanus toxoid conjugate as immunogen;Beuvery E. C.;Semin. Infect. Dis.,1982
4. Comparison of the induction of immunoglobulin M and G antibodies in mice with purified pneumococcal type 3 and meningococcal group C polysaccharides and their protein conjugates;Beuvery E. C.;Infect. Immun.,1982
5. Secondary IgG responses to type III pneumococcal polysaccharide. III. T-cell requirement for the development of B memory cells;Braley-Mullen H.;Eur. J. Immunol.,1977
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