Affiliation:
1. Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, Colorado 80045
Abstract
ABSTRACT
The disassembly of promoter nucleosomes appears to be a general property of highly transcribed eukaryotic genes. We have previously shown that the disassembly of chromatin from the promoters of the
Saccharomyces cerevisiae PHO5
and
PHO8
genes, mediated by the histone chaperone anti-silencing function 1 (Asf1), is essential for transcriptional activation upon phosphate depletion. This mechanism of transcriptional regulation is shared with the
ADY2
and
ADH2
genes upon glucose removal. Promoter chromatin disassembly by Asf1 is required for recruitment of TBP and RNA polymerase II, but not the Pho4 and Pho2 activators. Furthermore, accumulation of
SWI/SNF
and
SAGA
at the
PHO5
promoter requires promoter chromatin disassembly. By contrast, the requirement for
SWI/SNF
and
SAGA
to facilitate Pho4 activator recruitment to the nucleosome-buried binding site in the
PHO5
promoter occurs prior to chromatin disassembly and is distinct from the stable recruitment of
SWI/SNF
and
SAGA
that occurs after chromatin disassembly.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
80 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献