Molecular Karyotype of the White Rot Fungus Pleurotus ostreatus-Reference-Cited by-同舟云学术

Molecular Karyotype of the White Rot Fungus Pleurotus ostreatus

Published:1999-08 Issue:8 Volume:65 Page:3413-3417
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Larraya Luis M.¹,Pérez Gumer¹,Peñas María M.¹,Baars Johan J. P.²,Mikosch Thomas S. P.²,Pisabarro Antonio G.¹,Ramírez Lucía¹

Affiliation:

1. Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, Spain,1 and

2. Mushroom Experimental Station, NL-5960 AA Horst, The Netherlands2

Abstract

ABSTRACT The white rot fungus Pleurotus ostreatus is an edible basidiomycete with increasing agricultural and biotechnological importance. Genetic manipulation and breeding of this organism are restricted because of the lack of knowledge about its genomic structure. In this study, we analyzed the genomic constitution of P. ostreatus by using pulsed-field gel electrophoresis optimized for the separation of its chromosomes. We have determined that it contains 11 pairs of chromosomes with sizes ranging from 1.4 to 4.7 Mbp. In addition to chromosome separation, the use of single-copy DNA probes allowed us to resolve the ambiguities caused by chromosome comigration. When the two nuclei present in the dikaryon were separated by protoplasting, analysis of their karyotypes revealed length polymorphisms affecting various chromosomes. This is, to our knowledge, the clearest chromosome separation available for this species.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.8.3413-3417.1999

Reference43 articles.

1. Enzymatic Mechanisms Involved in Phenanthrene Degradation by the White Rot Fungus Pleurotus ostreatus

2. The mushroom Pleurotus ostreatus reduces secretion and accelerates the fractional turnover rate of very-low-density lipoproteins in the rat.;Bobek P.;Ann. Nutr. Metab.,1993

3. The mushroom Pleurotus ostreatus accelerates plasma very-low-density lipoprotein clearance in hypercholesterolemic rat.;Bobek P.;Physiol. Res.,1994

4. Chiu S.-W. Nuclear changes during fungal development Patterns in fungal development. Chiu S.-W. Moore D. 1996 105 125 Cambridge University Press Cambridge United Kingdom

5. A plant DNA minipreparation: version II.;Dellaporta S. L.;Plant Mol. Biol. Report,1983

Cited by 86 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The pathogenicity and regulatory function of temperature-sensitive proteins PscTSP in Pseudofabraea citricarpa under high temperature stress;International Journal of Biological Macromolecules;2024-06

2. Pleurotus ostreatus as a model mushroom in genetics, cell biology, and material sciences;Applied Microbiology and Biotechnology;2024-02-19

3. In silico analysis of the expression profile of AA9 Lytic Polysaccharide Monooxygenases (LPMOs) and the CDH Cellobiose Dehydrogenase enzyme in wood-degrader Agaricomycetes. The Pleurotus ostreatus case;Current Research in Biotechnology;2024

4. Altered Expression of Two Small Secreted Proteins (ssp4 and ssp6) Affects the Degradation of a Natural Lignocellulosic Substrate by Pleurotus ostreatus;International Journal of Molecular Sciences;2023-11-27

5. Visualizing organelles with recombinant fluorescent proteins in the white-rot fungus Pleurotus ostreatus;Fungal Biology;2023-10