Interlaboratory Optimization and Evaluation of a Serological Assay for Diagnosis of Human Baylisascariasis-Reference-Cited by-同舟云学术

Interlaboratory Optimization and Evaluation of a Serological Assay for Diagnosis of Human Baylisascariasis

Published:2013-11 Issue:11 Volume:20 Page:1758-1763
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Rascoe Lisa N.¹,Santamaria Cynthia²,Handali Sukwan¹,Dangoudoubiyam Sriveny³,Kazacos Kevin R.³,Wilkins Patricia P.¹,Ndao Momar²

Affiliation:

1. Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

2. National Reference Centre for Parasitology, Research Institute of the McGill University Health Centre, Montreal, Canada

3. Department of Comparative Pathobiology, Purdue University, West Lafayette, Indiana, USA

Abstract

ABSTRACT A Western blot assay using a recombinant protein, recombinant Baylisascaris procyonis RAG1 protein (rBpRAG1), was developed for the diagnosis of human baylisascariasis concurrently by the Centers for Disease Control and Prevention (CDC) in Atlanta, Georgia, and the National Reference Centre for Parasitology (NRCP) in Montreal, Canada. Assay performance was assessed by testing 275 specimens at the CDC and 405 specimens at the NRCP. Twenty specimens from 16 cases of baylisascariasis were evaluated. Eighteen were positive, with the assay correctly identifying 14 of 16 patients. The rBpRAG1 Western blot assay showed no cross-reactivity with Toxocara -positive serum and had an overall sensitivity of 88% and a specificity of 98%.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00387-13

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