Abstract
Complementation in bacteriophage E79 tv-l-mediated transduction and the phenotypic properties of the flagellar genes in Pseudomonas aeruginosa PAO were investigated by using 195 flagellar mutants of this organism. A total of 15 fla. 1 mot, and 2 che cistrons were identified. At least 5 fla cistrons (fla V to flaZ) and one mot cistron resided in one region, and at least 10 fla cistrons (flaA to flaJ) and two che cistrons (cheA and cheB) resided in another. The flaC mutants exhibited cistron-specific leakiness on motility agar plates. The flaE cistron may be the structural gene for the component protein of the flagellar filament. The cheA mutations, which resulted in pleiotropic phenotypes for flagellar formation, motility, and taxis, belonged to the same complementation group as the flaF mutations; that is, we inferred that cheA and flaF are synonymous.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference32 articles.
1. Adams M. H. 1959. Bacteriophages. Interscience Publishers Inc. New York.
2. Genetics of motility in Escherichia coli: complementation of paralysed mutants;Armstrong J. B.;Genetics,1967
3. Linkage map of Escherichia coli K-12, edition 6;Bachmann B. J.;Microbiol. Rev.,1980
4. Bacterial behaviour;Berg H. C.;Nature (London),1975
5. Buchanan R. E. and N. E. Gibbons (ed.). 1974. Bergey's manual of determinative bacteriology 8th ed. The Williams & Wilkins Co. Baltimore.
Cited by
16 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献