Affiliation:
1. Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann, D-1000 Berlin 33 (Dahlem), Federal Republic of Germany
Abstract
Escherichia coli
mutants with alterations in the electrophoretic mobility of ribosomal protein S9 were used to locate
rpsI
, the gene for this protein, on the linkage map.
rpsI
was located at about 70 min, roughly halfway between
argG
and
fabE
. It was very close to the gene for ribosomal protein L13,
rplM
. Another mutation at the
rpsI
locus gave rise to a phenotype of kasugamycin dependence and resistance. In this mutant, dependence on antibiotic came from kasugamycin being necessary to slow the rate of protein synthesis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference11 articles.
1. Linkage map of Escherichia coli K-12, edition 6;Bachmann B. J.;Microbiol. Rev.,1980
2. Mutational alterations in 50 proteins of the Escherichia coli ribosome;Dabbs E. R.;Mol. Gen. Genet.,1978
3. Kasugamycin-dependent mutants of Escherichia coli;Dabbs E. R.;J. Bacteriol.,1978
4. The gene for ribosomal protein S21, rpsU, maps close to dnaG at 66.5 min on the Escherichia coli chromosomal linkage map;Dabbs E. R.;J. Bacteriol.,1980
5. The gene for ribosomal protein L13, rp/M, is located near argR, at about 70 minutes on the Escherichia coli chromosomal linkage map;Dabbs E. R.;J. Bacteriol.,1982
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