Affiliation:
1. Federal Institute for Risk Assessment, National Salmonella Reference Laboratory, Berlin, Germany
2. Anagnostec GmbH, Am Mühlenberg 11, D-14476 Potsdam-Golm, Germany
Abstract
ABSTRACT
Variations in the mass spectral profiles of multiple housekeeping proteins of 126 strains representing
Salmonella enterica
subsp.
enterica
(subspecies I),
S. enterica
subsp.
salamae
(subspecies II),
S. enterica
subsp.
arizonae
(subspecies IIIa),
S. enterica
subsp.
diarizonae
(subspecies IIIb),
S. enterica
subsp.
houtenae
(subspecies IV), and
S. enterica
subsp.
indica
(subspecies VI), and
Salmonella bongori
were analyzed to obtain a phylogenetic classification of salmonellae based on whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometric bacterial typing. Sinapinic acid produced highly informative spectra containing a large number of biomarkers and covering a wide molecular mass range (2,000 to 40,000 Da). Genus-, species-, and subspecies-identifying biomarker ions were assigned on the basis of available genome sequence data for
Salmonella
, and more than 200 biomarker peaks, which corresponded mainly to abundant and highly basic ribosomal or nucleic acid binding proteins, were selected. A detailed comparative analysis of the biomarker profiles of
Salmonella
strains revealed sequence variations corresponding to single or multiple amino acid changes in multiple housekeeping proteins. The resulting mass spectrometry-based bacterial classification was very comparable to the results of DNA sequence-based methods. A rapid protocol that allowed identification of
Salmonella
subspecies in minutes was established.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
224 articles.
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