Affiliation:
1. Department of Laboratory Medicine, Bellin Memorial Hospital, Green Bay, Wisconsin 54305.
Abstract
Highly sensitive and rapid results can be obtained by isolating herpes simplex virus from clinical specimens in simple cell culture with rhabdomyosarcoma (RD) cells. In this study, 3,186 clinical specimens were inoculated into locally produced, equivalent-age RD and mink lung (ML) cells. Of 727 positive isolates, all (100%) were isolated from RD cells and only 691 (95%) were isolated from ML cells. Furthermore, 162 of the positive isolates (22%) were isolated in RD cells earlier than in ML cells. RD cells are continuous and can be cultivated in house without decreasing sensitivity as the passage number increases. They produce a highly distinguishable cytopathic effect in response to herpes simplex virus and maintain intense confirmatory staining patterns.
Publisher
American Society for Microbiology
Reference16 articles.
1. Comparison of the Ortho Cultureset (Vero cells) and Difco Cellmatics system (mink Iung and primary rabbit kidney cells) for the detection of herpes simplex virus from clinical specimens;Beckwith D. G.;Diagn. Microbiol. Infect. Dis.,1987
2. Butohd L. M. N. L. Schindler and H. W. Rogers. 1987. NOTES 2807
3. Drew W. L. and W. E. Rawls. 1985. Herpes simplex viruses p. 705-709. In E. H. Lennette A. Balows W. J. Hausler Jr. and H. J. Shadomy (ed.) Manual of clinical microbiology 4th ed. American Society for Microbiology Washington D.C.
4. Detection of herpes simplex virus in conventional tube cell cultures and in shell vials with a DNA probe kit and monoclonal antibodies;Espy M. J.;J. Clin. Microbiol.,1988
5. Evaluation of five cell types for the isolation of herpes simplex virus;Fayram S. L.;Diagn. Microbiol. Infect. Dis.,1986
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献