Author:
Spivak G,Ganesan A K,Hanawalt P C
Abstract
Irradiating the plasmid pSV2-gpt with UV (254 nm) doses up to 200 J m-2 caused a dose-dependent increase in the yield of Gpt+ transformants when the plasmid was introduced into human cells by calcium phosphate coprecipitation. UV doses greater than 1 kJ m-2 were required to reduce the efficiency of transformation below that obtained with unirradiated DNA.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
54 articles.
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