Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections-Reference-Cited by-同舟云学术

Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections

Published:2008-01 Issue:1 Volume:15 Page:95-100
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Marcilla A.¹²³⁴⁵,De la Rubia J. E.¹²³⁴⁵,Sotillo J.¹²³⁴⁵,Bernal D.¹²³⁴⁵,Carmona C.¹²³⁴⁵,Villavicencio Z.¹²³⁴⁵,Acosta D.¹²³⁴⁵,Tort J.¹²³⁴⁵,Bornay F. J.¹²³⁴⁵,Esteban J. G.¹²³⁴⁵,Toledo R.¹²³⁴⁵

Affiliation:

1. Área de Parasitología, Departamento de Biología Celular y Parasitología, Facultad de Farmacia, Universitat de València, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain

2. Departamento de Bioquímica y Biología Molecular, Facultat de Ciències Biològiques, Universitat de Valencia, Valencia, Spain

3. Unidad de Biología Parasitaria, Departamento de Biología Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay

4. División de Parasitología, Facultad de Farmacia, Universidad Miguel Hernández, Alicante, Spain

5. Departamento de Genética, Facultad de Medicina, Universidad de la República, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay

Abstract

ABSTRACT The liver fluke Fasciola hepatica parasitizes humans and ruminant livestock worldwide, and it is now being considered a reemerging zoonotic disease, especially in areas in which it is endemic, such as South America. This study investigates the immune response to excretory and secretory products produced by F. hepatica in a group of patients from the Peruvian Altiplano, where the disease is highly endemic. Using a proteomic approach and immunoblotting techniques, we have identified the enzymes leucine aminopeptidase (LAP) and phosphoenolpyruvate carboxykinase as immunodominant antigens recognized by sera from fasciolosis patients. An indirect enzyme-linked immunosorbent assay using recombinant LAP as the antigen was developed to check sera from individuals of this region. Our results demonstrate that LAP produces a specific and strong reaction, suggesting its potential use in the serologic diagnosis of F. hepatica infections in humans.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00338-07

Reference64 articles.

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