Affiliation:
1. Infectious Disease Division and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114-2696
Abstract
ABSTRACT
NorA, a multidrug efflux pump in
Staphylococcus aureus
, protects the cell from multiple drugs, including quinolones. The
flqB
mutation (T→G) in the 5′ untranslated region upstream of
norA
causes
norA
overexpression of 4.9-fold in
cis
, as measured in
norA
::
blaZ
fusions. The transcriptional initiation site of
norA
was unchanged in mutant and wild-type strains, but the half-life of
norA
mRNA was increased 4.8-fold in the
flqB
mutant compared to the wild-type strain. Computer-generated folding of the first 68 nucleotides of the
norA
transcript predicts an additional stem-loop and changes in a putative RNase III cleavage site in the
flqB
mutant.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
61 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献