Affiliation:
1. Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden
Abstract
ABSTRACT
The
trmD
operon is located at 56.7 min on the genetic map of the
Escherichia coli
chromosome and contains the genes for ribosomal protein (r-protein) S16, a 21-kDa protein (RimM, formerly called 21K), the tRNA (m
1
G37)methyltransferase (TrmD), and r-protein L19, in that order. Previously, we have shown that strains from which the
rimM
gene has been deleted have a sevenfold-reduced growth rate and a reduced translational efficiency. The slow growth and translational deficiency were found to be partly suppressed by mutations in
rpsM
, which encodes r-protein S13. Further, the RimM protein was shown to have affinity for free ribosomal 30S subunits but not for 30S subunits in the 70S ribosomes. Here we have isolated several new suppressor mutations, most of which seem to be located close to or within the
nusA
operon at 68.9 min on the chromosome. For at least one of these mutations, increased expression of the ribosome binding factor RbfA is responsible for the suppression of the slow growth and translational deficiency of a Δ
rimM
mutant. Further, the RimM and RbfA proteins were found to be essential for efficient processing of 16S rRNA.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference54 articles.
1. Mutant DnaK chaperones cause ribosome assembly defects in Escherichia coli;Alix J.-H.;Proc. Natl. Acad. Sci. USA,1993
2. RNA processing in prokaryotic cells;Apirion D.;Bioessays,1993
3. STUDIES ON LYSOGENESIS I
4. Modification of stable RNA;Björk G. R.;Escherichia coli and Salmonella typhimurium: cellular and molecular biology,1987
5. Transfer RNA modification;Björk G. R.;Annu. Rev. Biochem.,1987
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