An Autonomously Replicating Transforming Vector for Sulfolobus solfataricus-Reference-Cited by-同舟云学术

An Autonomously Replicating Transforming Vector for Sulfolobus solfataricus

Published:1998-06-15 Issue:12 Volume:180 Page:3237-3240
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Cannio Raffaele¹,Contursi Patrizia¹,Rossi Mosè²,Bartolucci Simonetta¹

Affiliation:

1. Dipartimento di Chimica Organica e Biologica, Università di Napoli, 16-80134 Naples,1and

2. Istituto di Biochimica delle Proteine ed Enzimologia del CNR, 10-80124 Naples,2 Italy

Abstract

ABSTRACT A plasmid able to transform and to be stably maintained both in Sulfolobus solfataricus and in Escherichia coli was constructed by insertion into an E. coli plasmid of the autonomously replicating sequence of the virus particle SSV1 and a suitable mutant of the hph (hygromycin phosphotransferase) gene as the transformation marker. The vector suffered no rearrangement and/or chromosome integration, and its copy number in Sulfolobus was increased by exposure of the cells to mitomycin C.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.180.12.3237-3240.1998

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