Affiliation:
1. Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Iseigaoka 1-1, Yahatanishiku, Kitakyushu 807-8555, Japan
Abstract
ABSTRACT
We have previously reported the isolation and characterization of two filamentous bacteriophages of
Vibrio parahaemolyticus
, designated Vf12 and Vf33. In this study, to understand the potential of these phages as tools for genetic transmission, we investigated the gene structures of replicative-form (RF) DNAs of their genomes and the distribution of these DNAs on chromosomal and extrachromosomal DNAs. The 7,965-bp nucleotide sequences of Vf12 and Vf33 were determined. An analysis of the overall gene structures revealed that Vf12 and Vf33 had conserved regions and distinctive regions. The gene organization of their conserved regions was similar to that of CTX phage of
Vibrio cholerae
and coliphage Ff of
Escherichia coli
, while their distinctive regions were characteristic of Vf12 and Vf33 phage genomes. Southern blot hybridization testing revealed that the filamentous phage genomes integrated into chromosomal DNA of
V. parahaemolyticus
at the distinctive region of the phage genome and were also distributed on some plasmids of
V. parahaemolyticus
and total cellular DNAs of one
Vibrio damsela
and one nonagglutinable
Vibrio
strain tested. These results strongly suggest the possibilities of genetic interaction among the bacteriophage Vf12 and Vf33 genomes and chromosomal and plasmid-borne DNAs of
V. parahaemolyticus
strains and of genetic transmission among strains through these filamentous phages.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
39 articles.
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