Menaquinone (Vitamin K 2 ) Biosynthesis: Localization and Characterization of the menA Gene from Escherichia coli

Author:

Suvarna K.1,Stevenson D.1,Meganathan R.1,Hudspeth M. E. S.1

Affiliation:

1. Department of Biological Sciences, Northern Illinois University, DeKalb, Illinois 60115

Abstract

ABSTRACT A key reaction in the biosynthesis of menaquinone involves the conversion of the soluble bicyclic naphthalenoid compound 1,4-dihydroxy-2-naphthoic acid (DHNA) to the membrane-bound demethylmenaquinone. The enzyme catalyzing this reaction, DHNA-octaprenyltransferase, attaches a 40-carbon side chain to DHNA. The menA gene encoding this enzyme has been cloned and localized to a 2.0-kb region of the Escherichia coli genome between cytR and glpK . DNA sequence analysis of the cloned insert revealed a 308-codon open reading frame (ORF), which by deletion analyses was shown to restore anaerobic growth of a menA mutant. Reverse-phase high-performance liquid chromatography analysis of quinones extracted from the orf -complemented cells independently confirmed the restoration of menaquinone biosynthesis, and similarly, analyses of isolated cell membranes for DHNA octaprenyltransferase activity confirmed the introduction of the menA product into the orf -complemented menA mutant. The validity of an ORF-associated putative promoter sequence was confirmed by primer extension analyses.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference25 articles.

1. COQ2 is a candidate for the structural gene encoding para-hydroxybenzoate:octaprenyltransferase;Asby M. N.;J. Biol. Chem.,1992

2. Ausubel F. M. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. Current protocols in molecular biology 1989 4.4.1 4.9.8 John Wiley and Sons New York N.Y

3. Biosynthesis of vitamin K and other natural naphthoquinones;Bentley R.;Pure Appl. Chem.,1975

4. Biosynthesis of vitamin K (menaquinone) in bacteria

5. A rapid alkaline extraction procedure for screening recombinant plasmid DNA

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3