Affiliation:
1. Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, Arizona 85721
Abstract
ABSTRACT
The 2.4-kb plasmid pAP1 from
Arcanobacterium
(
Actinomyces
)
pyogenes
had sequence similarity within the putative replication protein and double-stranded origin with the pIJ101/pJV1 family of plasmids. pJGS84, a derivative of pAP1 containing a kanamycin resistance gene, was able to replicate in
Escherichia coli
and
Corynebacterium pseudotuberculosis
, as well as in
A. pyogenes
. Detection of single-stranded DNA intermediates of pJGS84 replication suggested that this plasmid replicates by the rolling circle mechanism.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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