The MTCY428.08 Gene of Mycobacterium tuberculosis Codes for NAD + Synthetase-Reference-Cited by-同舟云学术

The MTCY428.08 Gene of Mycobacterium tuberculosis Codes for NAD + Synthetase

Published:1998-06-15 Issue:12 Volume:180 Page:3218-3221
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Cantoni Rita¹,Branzoni Manuela¹,Labò Monica¹,Rizzi Menico¹²,Riccardi Giovanna¹

Affiliation:

1. Dipartimento di Genetica e Microbiologia, Università di Pavia, 27100 Pavia,1and

2. Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, 10125 Torino,2 Italy

Abstract

ABSTRACT The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD + synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S -transferase fused and His 6 -tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD + synthetase.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.180.12.3218-3221.1998

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