Affiliation:
1. Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019-0245
Abstract
ABSTRACT
We compared the cellular fatty acid profiles of
Myxococcus xanthus
cells grown in either a Casitone-based complex medium or a chemically defined medium. The cells grown in the complex medium had a much higher content of the abundant branched-chain fatty acid
iso
-15:0 and several other branched-chain species. The higher branched-chain fatty acid content of the cells grown in the complex medium was dependent on the
esg
locus, which encodes the E1α and E1β components of a branched-chain keto acid dehydrogenase (BCKAD) multienzyme complex involved in branched-chain fatty acid biosynthesis. Cells grown in the complex medium were also found to have a higher level of
esg
transcription and more BCKAD enzyme activity than cells from the chemically defined medium. The level of
esg
transcription appears to be an important factor in the growth medium-dependent regulation of the
M. xanthus
branched-chain fatty acid content.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
7 articles.
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