Identification and Expression of the Bacillus subtilis Fructose-1,6-Bisphosphatase Gene ( fbp )

Author:

Fujita Yasutaro1,Yoshida Ken-Ichi1,Miwa Yasuhiko1,Yanai Nobuo1,Nagakawa Eishi1,Kasahara Yasuhiro2

Affiliation:

1. Department of Biotechnology, Faculty of Engineering, Fukuyama University, Fukuyama 729-0292,1 and

2. Graduate School of Biological Sciences, Nara Advanced Institute of Science and Technology, Ikoma 630-0101,2 Japan

Abstract

ABSTRACT The Bacillus subtilis fbp gene encoding fructose-1,6-bisphosphatase (FBPase) was originally identified as yydE . The fbp gene was expressed at a fairly constant level in cells undergoing glycolysis or gluconeogenesis. fbp transcription was initiated 94 bp upstream of the translation initiation codon, resulting in a 2.4-kb monocistronic transcript. Interestingly, B. subtilis FBPase exhibited no significant similarity to other FBPases in protein sequence databases.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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