Affiliation:
1. Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom
Abstract
ABSTRACT
A DNA locus from
Bordetella pertussis
capable of reconstituting lipopolysaccharide (LPS) O-antigen biosynthesis in
Salmonella typhimurium
SL3789 (
rfaF511
) has been isolated, by using selection with the antibiotic novobiocin. DNA within the locus encodes a protein with amino acid sequence similarity to heptosyltransferase II, encoded by
waaF
(previously
rfaF
) in other gram-negative bacteria. Mutation of this gene in
B. pertussis
,
Bordetella parapertussis
, and
Bordetella bronchiseptica
by allelic exchange generated bacteria with deep rough LPS phenotypes consistent with the proposed function of the gene as an inner core heptosyltransferase. These are the first LPS mutants generated in
B. parapertussis
and
B. bronchiseptica
and the first deep rough mutants of any of the bordetellae.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
32 articles.
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