Simple in vitro translation assay to analyze inhibitors of rhinovirus proteases-Reference-Cited by-同舟云学术

Simple in vitro translation assay to analyze inhibitors of rhinovirus proteases

Published:1996-01 Issue:1 Volume:40 Page:267-270
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Heinz B A¹,Tang J¹,Labus J M¹,Chadwell F W¹,Kaldor S W¹,Hammond M¹

Affiliation:

1. Infectious Disease Research, Lilly Research Laboratories, Indianapolis, Indiana 46285, USA.

Abstract

We have developed a simple in vitro translation method to analyze compounds that inhibit the rhinovirus 3C protease in peptide substrate assays but demonstrate no antiviral activity. This complementary assay, which provides both qualitative and quantitative results, detects the inhibition of the 3CD protease in the native polyprotein form.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.40.1.267

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